Hello, usually you always count on c/c between the joists and set it as the length. But yes theoretically it has some impact, but then it would probably require that there is flooring on both sides of the joist that loads the edges equally, otherwise the joist will get an uneven load and then the load will creep towards the center joist since the joist is usually not very torsionally rigid.
But as an example, if you have a 45-rule on c/c 600 mm with flooring that lies on the edge of the rule instead of the center, the difference in deflection should be L1^4/L2^4=600^4/(600-45)^4=1.37, so 37% greater deflection with a span of 600 mm than 555 mm.